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Other than presumed in the thread title, I don't expect an "antenna trace" problem. Trace impedance is at least near to 50 ohms according to PCB parameters. It's more likely an antenna or  software configuration problem. Using a pair of devices under test is possible for verfication, but no good way to debug the design. Spectrum analyzer measurements would be my first step to verify transmitter operation, checking output power, frequency, modulation and spurious emissions. It can be also used for qualitative check of radiated field strength in combination with an antenna of known antenna factor, e.g a lambda/2 dipole. Consider that attenuators (20 - 40 dB) are required for measurements at antenna output.