omore is correct, it depends on the circuit you use as to whether or not the secondary (of a transformer) is grounded. At the very least, the chassis is connected to the ground prong on the power outlet on most equipment. On some power supplies, the secondary is floating. On others, the secondary is grounded at some point. This is good information to know, because it affects whether or not you can put the supplies in series. Generally, I assume the zero volt terminal is grounded and avoid using supplies in series. You can check this using an ohmmeter to check for connection between the ground plug and the zero volt terminal. You can isolate the terminal from ground (sometimes there is a wire from the secondary to the chassis, sometimes its done on the board itself, and sometimes AT THE TERMINAL ITSELF). If you disconnect the ground, you will still have rated voltage between the terminals in circuits that use a transformer with the secondary isolated from the primary, but the zero voltage terminal may not be at ground potential anymore.
Some power supplies do not use a transformer. I don't think you can isolate those.