I would like to ask if there is a difference in the layout between 3.3 and 5V MOS. Is thick active layer involved here?
And if we can use 3.3 and 5V MOS devices in a single chip. Thank you!
Yes, in the same process, i.e. on the same chip. However, usually two different gate oxide thicknesses are used for 3.3 and 5V, i.e an option which needs -at least- two more masks. Moreover, the 5V MOSFETs must have larger W and L dimensions than the process' minimum.
No. Active actually isn't a layer, these are etched (and, mostly, implant-doped) holes in the field oxide, defining the transistor bulk areas - both the same field oxide openings for 3.3 and 5V transistors, but separate active areas for both types.