im into amplifing biosignals too, im simulating with ac waveforms ( one with 12 mv peak, the other with 10 mv peak) with two generators and i calculated a Gain of 1000 but i got 2000 instead 1000, furthermore i have not offset, any idea why of this?
There is probably a mistake in your circuit or in your calculation.
If you want somebody to help you find the problem, you need to show a picture of the circuit.