help with absolute number of DC subcarrier

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Hello all,
I have question for OFDM, how to decide which sub-carriers should I allocate it for the DC, should be in the centre to keep the symmetrical alignment of sub-carriers or on the Edge if we assume that I am using 256 IFFt length and only 225 sub-carriers for data and without using pilot.
if the number of data sub-carriers is 225, I'll get 31 nulls. So, where they should be mapped?

Also, how to calculate sampling frequency of the multipath channel in MHz and can I obtain the sub-carriers frequencies from it or it needs another calculation?

helps appreciated.
Best,
 
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