every thing about instrumentation amplifier
The circuit can work without negative supply. As a main restriction, it has only unipolar output (e.g. positive sensor currents)
and it can't measure values near zero. If these restriction are acceptable, the circuit is O.K.in my opinion.
I understand, that the output is intended to drive a PIC ADC (0..5V range), so negative outputs won't be meaningful anyway.
You may want to adjust the reference voltage to include the sensor zero in the observable measurement range.
The reason for the said "gain fall" can't be seen from the circuit, I think. You should mention the connected LEM sensor type,
but common types should work with the circuit. Most likely there's a problem that's hidden in your circuit (e.g. missing ground
connection, or similar).