yuo add it where the parts of very most different voltages are near each other.
Maybe you check it first b4 give to UL
...you hold the trafo over a steaming kettle for a few minutes, then do a flash test and see if/where it flashes.
..put tube where it flashed........just put tube anyway, to make it look like you care.
Search under "conductor spacing distances", conductor creepage distance" , conductor clearance distances, "PCB layout rules for track spacing" etc etc