No most of the people tell to provide reference plane. why is important question.. Differential signaling is a method of transmitting information electrically with two complementary signals sent on two paired wires, called a differential pair. Since external interference tend to affect both wires together, and information is sent only by the difference between the wires, the technique improves resistance to electromagnetic noise compared with use of only one wire and an un-paired reference (ground). The technique can be used for both analog signaling, as in balanced audio, and digital signaling, as in RS-422, RS-485, Ethernet over twisted pair, PCI Express, DisplayPort, HDMI and USB. The opposite technique is called single-ended signaling. At the end of the connection, the receiving device reads the difference between the two signals. Since the receiver ignores the wires' voltages with respect to ground, small changes in ground potential between transmitter and receiver do not affect the receiver's ability to detect the signal.(with the help of Wiki).