Hi
Before you do any simulations I think you need to understand how the circuit works. In this case how the differential amplifier, and how MOS transistors work. If you are a self-taught and you need some guidance there are a few great books for beginners: "Analysis and Design of Analog Integrated Circuis - P. R. Gray" , "Desing of Analog CMOS Integrated Cirbuits - B. Razavi", "Microelectronic Circuits - Sedra", "Analog Integrated Circuit Design - Kenneth Martin" and may others.
If you are a student you can learn a lot of things by paying attention to class.
If you desing a differential amplifier like the one in your picture, you must make sure that the transistors work in saturation. If we asume that they obey the simple square law, this happens when the two following conditions are met:
1) The drain voltage is larger than the gate voltage - threshold voltage (VD>VG-Vth).
2) The gate-source voltage is greater than the treshold voltage (VGS > Vth).
Since Vth of an NMOS transistor is positive (assuming it is an enhancement mode MOS) it is clear that when you ground the imputs the circuit will not work as a differential amplifier. The transistors will be blocked.
A common method is to bias the gates of the input transistors to half the supply voltage - (VDD-VSS)/2. Since in your picture VSS=0 The input voltage will be VDD/2, but you have to make sure that the transistors remain in saturation.