Reply to thread

Dear Sir.

Thanks a lot for the reply.

I have few confusion regarding this that's why scare to apply the multiple frequencies.

1) there could be joule heating effect happen inside the channel.

2) there is break down of electrode could happen because of over voltage apply.

That's why i am using the constant 500vM RMS voltage. we actually apply the the RMS voltage using impedance analyzer, so for this reason i scare to change the voltage, but i will look for this option as well.

[automerge]1723651712[/automerge]

Dear Sir.

I am going to attach the calculation page, in case of some problem in my calculation, please tell me.

If this is the case if will check the results at each frequency less than 1kHz and greater 100Hz

[automerge]1723652579[/automerge]

Dear Klaus.

I am going to provide the complete information.

I have a sensor made from a microfluidic device with a channel inside it. At the bottom of the channel, there are electrodes to which I will send the signal.

Initially, when I flow the antibody through the channel, the antibody attaches to the electrodes surface on the bottom of the channel, resulting in an impedance value of 8-11 MΩ. After some time, I flow the sample into the channel. The sample again attaches to the electrode surface, causing the impedance value to rise to 25-28 MΩ.

I need to provide a signal with an amplitude of 500 mV. However, frequency-wise, I will use two different values. This is not the sweeping of frequency, but taking measurement at two different frequencies

1. 100 Hz
2. 1 kHz

I will take measurements at these two frequency points at different time intervals. I will not sweep the frequency from 100 Hz to 1 kHz, and I need to measure the phase angle or other parameters as well while in the impedance measurement.

I hope this clarifies the situation. If you have any further questions, please feel free to ask. Your comments are very valuable to me.

I am going to attach the pictures of impedance analyzer during taking the reading at antibody and sample flow and on the bases of these reading of impedance analyzer, i did the calculation for capacitance and resistance of the sensor, if this calculation is wrong kindly let me know.

I used the -70 angle for reference,  the angle remains between -68 to -72

[automerge]1723653693[/automerge]

Dear Sir.

Thanks a lot for the reply.

Yes i have cleared all the confusion.

Thanks again

[automerge]1723653844[/automerge]

Dear Sir,

I have attached the impedance analyzer results as well in the comments during the time of antibody flow and sample flow, You can verify the results of impedance and their phase as well over there in the comments sections.

I will take the measurement at two different frequency values, 100Hz  and 1Khz.

it would not be sweeping of frequency